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colorectal cancer cell lines hct116  (ATCC)


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    ATCC colorectal cancer cell lines hct116
    Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and <t>HCT116</t> cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.
    Colorectal Cancer Cell Lines Hct116, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 17934 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/colorectal+cancer+cell+lines+hct+116/HCT+116/pmc13155016-39-13-18
    Average 99 stars, based on 17934 article reviews
    colorectal cancer cell lines hct116 - by Bioz Stars, 2026-09
    99/100 stars

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    1) Product Images from "MBD2 suppresses SFRP1 expression and promotes colorectal cancer development by blocking MED19 binding to its methylated promoter"

    Article Title: MBD2 suppresses SFRP1 expression and promotes colorectal cancer development by blocking MED19 binding to its methylated promoter

    Journal: Cancer Biology & Therapy

    doi: 10.1080/15384047.2026.2667568

    Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and HCT116 cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.
    Figure Legend Snippet: Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and HCT116 cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.

    Techniques Used: Inhibition, Expressing, Activation Assay, Binding Assay, Concentration Assay

    The effect of KCC07 on recovering SFRP1 expression requires MED19. (A and B) The interfering efficiency of MED19 siRNAs at the protein level in SW480 and HCT116 cells. (C–F) Treatment with KCC07 resulted in increased SFRP1 expression and increased β -catenin expression at both the mRNA and protein levels in CRC cells, while simultaneous knockdown of MED19 abolished these effects. n = 6, ns indicates not significant, *indicates p < 0.05, and **indicates p < 0.01.
    Figure Legend Snippet: The effect of KCC07 on recovering SFRP1 expression requires MED19. (A and B) The interfering efficiency of MED19 siRNAs at the protein level in SW480 and HCT116 cells. (C–F) Treatment with KCC07 resulted in increased SFRP1 expression and increased β -catenin expression at both the mRNA and protein levels in CRC cells, while simultaneous knockdown of MED19 abolished these effects. n = 6, ns indicates not significant, *indicates p < 0.05, and **indicates p < 0.01.

    Techniques Used: Expressing, Knockdown

    Related Articles

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    Article Title: Mechanisms of diterpenes from Scutellaria Barbata D. Don against colorectal cancer via network pharmacology and experimental verification
    Article Snippet: The American Type Culture Collection (ATCC) supplied human colorectal cancer cell lines HCT-116 and FHC).

    Article Title: Integrative analysis of ubiquitination-related genes identifies HSPA1A as a critical regulator in colorectal cancer progression
    Article Snippet: Colorectal cancer cell lines HCT-116 and DLD1, sourced from the American Type Culture Collection (ATCC), were utilized in this study.

    Article Title: Combinational Synergistic Role of Thymoquinone and Celastrol in Colon Carcinoma Cell Line
    Article Snippet: Colorectal cancer cell lines (HCT-116) (ATCC-CCL-247) were obtained from the American Type Culture Collection in Virginia, USA.

    Article Title: Integrative analysis of ubiquitination-related genes identifies HSPA1A as a critical regulator in colorectal cancer progression.
    Article Snippet: Colorectal cancer cell lines HCT-116 and DLD1, sourced from the American Type Culture Collection (ATCC), were utilized in this study.

    Article Title: Nanotechnology-Driven Colorectal Cancer Treatment: Coencapsulation of Irinotecan and Cyclosporine A in SNEDDS for Superior Outcomes.
    Article Snippet: Irinotecan (IRN), a camptothecin derivative, has limited and inadequate oral absorption due to efflux pump activity by intestinal Pglycoprotein receptors.. To complete these challenges, we designed and fabricated irinotecan and cyclosporine.. A coloaded self-nanoemulsifying drug delivery system (CO-IR-CP-SNs) can effectively prevent P-gp efflux and P450 enzyme metabolization, increasing oral bioavailability.

    Article Title: Integrative analysis of recurrence related gene signature and STC1 in colorectal cancer proliferation and metastasis
    Article Snippet: The human colorectal cancer cell lines HCT-116 and DLD1 were obtained from the American Type Culture Collection (ATCC).

    Article Title: Design, synthesis and biological evaluation of 2-phenylquinoxaline carbonyl piperazine derivatives as novel FASN inhibitors with anticancer activity.
    Article Snippet: Overexpression of fatty acid synthase (FASN) has been linked to the advancement of various cancers.. FASN caters to the increased demand for lipids within tumor cells, facilitating tumor growth and progression, making it an attractive target for anticancer drug discovery.. Herein we report a novel series of 2-phenylquinoxaline-6-carboxylic acid derivatives as novel potent FASN inhibitors with anticancer potential.

    Cell Culture:

    Article Title: Colorectal Cancer Remains A Formidable Global Health Challenge
    Article Snippet: .. Cell culture The human colorectal cancer cell lines HCT-116 and DLD1 were obtained from the American Type Culture Collection (ATCC). ..



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    ATCC colorectal cancer cell lines hct116
    Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and <t>HCT116</t> cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.
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    Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and <t>HCT116</t> cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.
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    Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and <t>HCT116</t> cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.
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    Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and <t>HCT116</t> cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.
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    Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and <t>HCT116</t> cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.
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    scRNA-seq analysis of cell heterogeneity during <t>colorectal</t> carcinogenesis. (A) UMAP visualization of cell clusters from para-cancer, adenomatous polyp, and cancer tissues, and cancer tissues. A total of 15 independent cell clusters were identified. (B) Bubble plot of canonical marker gene expression for each cell clusters. (C) Heatmap showing the expression scores of the key WGCNA module genes across all identified cell clusters. Higher expression levels were predominantly observed in specific cell clusters, suggesting cell type-specific enrichment of these co-expression modules. (D–H) Violinplot showing the expression trends of module genes in corresponding cells.
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    scRNA-seq analysis of cell heterogeneity during <t>colorectal</t> carcinogenesis. (A) UMAP visualization of cell clusters from para-cancer, adenomatous polyp, and cancer tissues, and cancer tissues. A total of 15 independent cell clusters were identified. (B) Bubble plot of canonical marker gene expression for each cell clusters. (C) Heatmap showing the expression scores of the key WGCNA module genes across all identified cell clusters. Higher expression levels were predominantly observed in specific cell clusters, suggesting cell type-specific enrichment of these co-expression modules. (D–H) Violinplot showing the expression trends of module genes in corresponding cells.
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    Image Search Results


    Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and HCT116 cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.

    Journal: Cancer Biology & Therapy

    Article Title: MBD2 suppresses SFRP1 expression and promotes colorectal cancer development by blocking MED19 binding to its methylated promoter

    doi: 10.1080/15384047.2026.2667568

    Figure Lengend Snippet: Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and HCT116 cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.

    Article Snippet: Normal colon mucosa cell line NCM460 (ATCC, CRL-1642 TM ) and the human colorectal cancer cell lines HCT116 (ATCC, CCL-247 TM ) and SW480 (ATCC, CCL-228 TM ) were purchased from iCell, and all had STR identification reports.

    Techniques: Inhibition, Expressing, Activation Assay, Binding Assay, Concentration Assay

    The effect of KCC07 on recovering SFRP1 expression requires MED19. (A and B) The interfering efficiency of MED19 siRNAs at the protein level in SW480 and HCT116 cells. (C–F) Treatment with KCC07 resulted in increased SFRP1 expression and increased β -catenin expression at both the mRNA and protein levels in CRC cells, while simultaneous knockdown of MED19 abolished these effects. n = 6, ns indicates not significant, *indicates p < 0.05, and **indicates p < 0.01.

    Journal: Cancer Biology & Therapy

    Article Title: MBD2 suppresses SFRP1 expression and promotes colorectal cancer development by blocking MED19 binding to its methylated promoter

    doi: 10.1080/15384047.2026.2667568

    Figure Lengend Snippet: The effect of KCC07 on recovering SFRP1 expression requires MED19. (A and B) The interfering efficiency of MED19 siRNAs at the protein level in SW480 and HCT116 cells. (C–F) Treatment with KCC07 resulted in increased SFRP1 expression and increased β -catenin expression at both the mRNA and protein levels in CRC cells, while simultaneous knockdown of MED19 abolished these effects. n = 6, ns indicates not significant, *indicates p < 0.05, and **indicates p < 0.01.

    Article Snippet: Normal colon mucosa cell line NCM460 (ATCC, CRL-1642 TM ) and the human colorectal cancer cell lines HCT116 (ATCC, CCL-247 TM ) and SW480 (ATCC, CCL-228 TM ) were purchased from iCell, and all had STR identification reports.

    Techniques: Expressing, Knockdown

    scRNA-seq analysis of cell heterogeneity during colorectal carcinogenesis. (A) UMAP visualization of cell clusters from para-cancer, adenomatous polyp, and cancer tissues, and cancer tissues. A total of 15 independent cell clusters were identified. (B) Bubble plot of canonical marker gene expression for each cell clusters. (C) Heatmap showing the expression scores of the key WGCNA module genes across all identified cell clusters. Higher expression levels were predominantly observed in specific cell clusters, suggesting cell type-specific enrichment of these co-expression modules. (D–H) Violinplot showing the expression trends of module genes in corresponding cells.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Bioinformatics combined with machine learning for the identification of malignant transformation markers in colorectal polyps

    doi: 10.3389/fmolb.2026.1785464

    Figure Lengend Snippet: scRNA-seq analysis of cell heterogeneity during colorectal carcinogenesis. (A) UMAP visualization of cell clusters from para-cancer, adenomatous polyp, and cancer tissues, and cancer tissues. A total of 15 independent cell clusters were identified. (B) Bubble plot of canonical marker gene expression for each cell clusters. (C) Heatmap showing the expression scores of the key WGCNA module genes across all identified cell clusters. Higher expression levels were predominantly observed in specific cell clusters, suggesting cell type-specific enrichment of these co-expression modules. (D–H) Violinplot showing the expression trends of module genes in corresponding cells.

    Article Snippet: Human colorectal cancer cell lines (HCT116, LOVO, and RKO) were purchased from the ATCC, and the normal human colon mucosal epithelial cells (NCM460) was obtained from Yuchun Biotechnology Co., Ltd. (Shanghai, China).

    Techniques: Marker, Gene Expression, Expressing

    Expression and survival analysis of malignant transformation markers. (A) Protein expression profiles of these genes in normal and colorectal tumor tissues were inferred using the UALCAN online platform. (B) Kaplan–Meier survival curves illustrating the correlation between gene expression levels and overall survival of CRC patients. The x-axis represents time in months, and the y-axis represents the percentage of surviving patients. The red line represents patients with high gene expression, while the black line represents patients with low gene expression.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Bioinformatics combined with machine learning for the identification of malignant transformation markers in colorectal polyps

    doi: 10.3389/fmolb.2026.1785464

    Figure Lengend Snippet: Expression and survival analysis of malignant transformation markers. (A) Protein expression profiles of these genes in normal and colorectal tumor tissues were inferred using the UALCAN online platform. (B) Kaplan–Meier survival curves illustrating the correlation between gene expression levels and overall survival of CRC patients. The x-axis represents time in months, and the y-axis represents the percentage of surviving patients. The red line represents patients with high gene expression, while the black line represents patients with low gene expression.

    Article Snippet: Human colorectal cancer cell lines (HCT116, LOVO, and RKO) were purchased from the ATCC, and the normal human colon mucosal epithelial cells (NCM460) was obtained from Yuchun Biotechnology Co., Ltd. (Shanghai, China).

    Techniques: Expressing, Transformation Assay, Gene Expression